7UDN

Crystal structure of designed helical repeat protein RPB_PLP1_R6 in alternative conformation 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 2 21 21
Unit cell lengths	80.430, 86.480, 110.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.240 - 2.450
R-factor	0.2363
Rwork	0.234
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	predicted model
RMSD bond length	0.002
RMSD bond angle	0.339
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.240	2.510
High resolution limit [Å]	2.450	2.450
Rmeas	0.070	1.850
Number of reflections	28936	2101
<I/σ(I)>	14
Completeness [%]	99.9	100
Redundancy	6.6	6.7
CC(1/2)	1.000	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	291	40% v/v MPD, 0.1 M sodium phosphate-citrate, pH 4.2