7UDM
Crystal structure of designed helical repeat protein RPB_PLP1_R6 in alternative conformation 1 (with peptide)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 54.380, 80.260, 154.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.220 - 2.650 |
| R-factor | 0.2623 |
| Rwork | 0.260 |
| R-free | 0.29910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | predicted model |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.294 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.220 | 2.720 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmeas | 0.100 | 2.110 |
| Number of reflections | 20350 | 1492 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.7 | 6.7 |
| CC(1/2) | 1.000 | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.02 M calcium chloride, 30% v/v MPD, 0.1 M sodium acetate, pH 4.6 |
