7UDI

Full-length dimer of DNA-Damage Response Protein C from Deinococcus radiodurans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 41
Unit cell lengths	66.698, 66.698, 129.581
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.300 - 2.240
R-factor	0.2314
Rwork	0.231
R-free	0.24980
Structure solution method	SAD
RMSD bond length	0.021
RMSD bond angle	1.751
Data reduction software	autoPROC (1.0.5)
Data scaling software	autoPROC (1.0.5)
Phasing software	AutoSol (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.698	2.278
High resolution limit [Å]	2.239	2.239
Rmerge	0.055	0.706
Rmeas	0.073	0.899
Rpim	0.023	0.392
Number of reflections	26343	1335
<I/σ(I)>	19.1	2.3
Completeness [%]	96.8	99.9
Redundancy	10.1	5.2
CC(1/2)	0.998	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	250 uM DdrC 140 mM Sodium sulfate 14 mM Sodium citrate 3.6% (w/v) PEG 8000 4.3% (v/v) Pentaerythritol ethoxylate (3/4 EO/OH) 18 mM HEPES/NaOH, pH 7.5