7UD1
The Crystal Structure of Apo Monomer F57:H:H:H:R58 HCRBPII with Histidine Insertion in the Hinge Loop Region at 1.3 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-31 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.525, 47.815, 53.223 |
| Unit cell angles | 90.00, 92.61, 90.00 |
Refinement procedure
| Resolution | 29.090 - 1.320 |
| R-factor | 0.1876 |
| Rwork | 0.187 |
| R-free | 0.21250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rcq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.802 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.090 | 2.035 |
| High resolution limit [Å] | 1.320 | 1.964 |
| Rmerge | 0.062 | 0.621 |
| Rmeas | 0.087 | 0.602 |
| Number of reflections | 55379 | 5345 |
| <I/σ(I)> | 27.9 | 2.14 |
| Completeness [%] | 96.6 | 93.5 |
| Redundancy | 6.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 4000, Sodium acetate, ammonium acetate |
