7UD0
Room Temperature Drosophila Cryptochrome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2021-04-12 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.1271 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.000, 123.000, 81.000 |
| Unit cell angles | 90.00, 116.00, 90.00 |
Refinement procedure
| Resolution | 19.840 - 3.500 |
| Rwork | 0.251 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gu5 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.586 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.840 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.649 | 2.520 |
| Rmeas | 0.658 | 2.550 |
| Number of reflections | 14881 | 1462 |
| <I/σ(I)> | 5.9 | 2 |
| Completeness [%] | 89.8 | 89.1 |
| Redundancy | 47.4 | 48.6 |
| CC(1/2) | 0.269 | 0.213 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 8.5 | 298 | 5 mg/mL twin-strep tagged dCRY (1-539) 100 mM Tris (pH 8.5) 150 mM Magnesium acetate tetrahydrate 18% PEG-4000 Mixed 1:1 in Eppendorf tube; no vortexing |
