7UD0

Room Temperature Drosophila Cryptochrome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	298
Detector technology	PIXEL
Collection date	2021-04-12
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.1271
Spacegroup name	P 1 21 1
Unit cell lengths	74.000, 123.000, 81.000
Unit cell angles	90.00, 116.00, 90.00

Refinement procedure

Resolution	19.840 - 3.500
Rwork	0.251
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gu5
RMSD bond length	0.004
RMSD bond angle	0.586
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.840	3.630
High resolution limit [Å]	3.500	3.500
Rmerge	0.649	2.520
Rmeas	0.658	2.550
Number of reflections	14881	1462
<I/σ(I)>	5.9	2
Completeness [%]	89.8	89.1
Redundancy	47.4	48.6
CC(1/2)	0.269	0.213

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	8.5	298	5 mg/mL twin-strep tagged dCRY (1-539) 100 mM Tris (pH 8.5) 150 mM Magnesium acetate tetrahydrate 18% PEG-4000 Mixed 1:1 in Eppendorf tube; no vortexing