7UCZ
The Crystal Structure of Apo Monomer F57:H:H:H:H:H:H:R58 Mutant of HCRBPII with Histidine Insertion in the Hinge Loop Region at 1.1 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.608, 36.931, 68.513 |
| Unit cell angles | 73.41, 83.92, 66.06 |
Refinement procedure
| Resolution | 27.060 - 1.080 |
| R-factor | 0.2002 |
| Rwork | 0.200 |
| R-free | 0.21310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rcq |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.744 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.060 | 1.100 |
| High resolution limit [Å] | 1.079 | 1.080 |
| Rmerge | 0.060 | 0.507 |
| Rmeas | 0.079 | 0.593 |
| Number of reflections | 101925 | 9597 |
| <I/σ(I)> | 33.3 | 3.3 |
| Completeness [%] | 93.4 | 89.4 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | PEG 4000, sodium acetate, ammonium acetate |
