7UCY

Integrin alaphIIBbeta3 complex with gantofiban

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.963
Spacegroup name	P 21 21 2
Unit cell lengths	258.550, 144.460, 105.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.980 - 2.350
R-factor	0.221398991762
Rwork	0.221
R-free	0.23081
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t3p
RMSD bond length	0.007
RMSD bond angle	0.576
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.410
High resolution limit [Å]	2.350	2.350
Number of reflections	315839	23370
<I/σ(I)>	3.78
Completeness [%]	99.7
Redundancy	2.14
CC(1/2)	0.976	0.111

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.9	277	11% PEG 8000, 0.2 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.9