7UCS

The Crystal Structure of Domain-Swapped Dimer Q108K:T51D:A28C:L36C:F57:H:H:H:R58 Mutant of hCRBPII with Histidine Insertion in the Hinge Loop Region at 1.92 Angstrom Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-04
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.97626
Spacegroup name	P 21 21 21
Unit cell lengths	36.929, 69.982, 106.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.890 - 1.920
R-factor	0.1986
Rwork	0.195
R-free	0.22920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rcq
RMSD bond length	0.007
RMSD bond angle	0.842
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.890	1.950
High resolution limit [Å]	1.920	1.920
Rmerge	0.073	0.686
Number of reflections	21404	2032
<I/σ(I)>	21.7	2.4
Completeness [%]	98.9	99.8
Redundancy	11.8	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Sodium acetate, PEG4000, ammonium acetate