7UCC

Transcription factor FosB/JunD bZIP domain in the reduced form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-09-10
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	47.939, 70.244, 122.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.230 - 1.940
R-factor	0.2077
Rwork	0.204
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vpf poly-alanine
Data reduction software	HKL-2000 (2.3.15)
Data scaling software	HKL-2000 (2.3.15)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	33.230	1.970
High resolution limit [Å]	1.940	5.260	1.940
Rmerge	0.066	0.042	0.328
Rmeas	0.070	0.044	0.352
Rpim	0.020	0.013	0.120
Number of reflections	13357	876	194
<I/σ(I)>	9.9
Completeness [%]	84.1	99.4	25.1
Redundancy	10.8	12.3	5.8
CC(1/2)	0.976	0.999	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	100 mM Tris, pH 7.0, 40% (v/v) ethanol