7UBZ
Chymotrypsin digested toxin/immunity complex for a T6SS lipase effector from E. cloacae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.172, 120.493, 122.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.760 - 1.750 |
| R-factor | 0.1666 |
| Rwork | 0.164 |
| R-free | 0.18820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7UC0 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.19.1-4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.880 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.119 | 2.725 |
| Rmeas | 0.121 | 2.772 |
| Rpim | 0.020 | 0.497 |
| Total number of observations | 4829779 | |
| Number of reflections | 130201 | 5526 |
| <I/σ(I)> | 23.2 | 2.3 |
| Completeness [%] | 99.1 | 85.9 |
| Redundancy | 37.1 | 29.3 |
| CC(1/2) | 1.000 | 0.733 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.25 M sodium citrate, 22% PEG3350 |
