7U89

Product of 14mer primer with activated G monomer diastereomer 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	98
Detector technology	CCD
Collection date	2021-05-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 3 2 1
Unit cell lengths	43.204, 43.204, 85.638
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.562 - 1.650
Rwork	0.216
R-free	0.28160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6c8o
RMSD bond length	0.021
RMSD bond angle	2.984
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.650
High resolution limit [Å]	1.650	1.650
Rmerge	0.086	0.260
Rmeas	0.091	0.277
Rpim	0.029	0.093
Number of reflections	11662	553
<I/σ(I)>	21	5.6
Completeness [%]	99.6	98.9
Redundancy	10.1	7.8
CC(1/2)	0.989	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	20 mM Magnesium chloride, 50 mM MOPS pH 7.0, 2.0 M Ammonium sulfate, 0.5 mM Spermine