7U7Y

Human DNA polymerase eta-DNA-dGMPNPP ternary mismatch complex in 0.06 mM Mn2+ for 600s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-03-31
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9786
Spacegroup name	P 61
Unit cell lengths	98.607, 98.607, 81.801
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.700 - 1.780
R-factor	0.182
Rwork	0.179
R-free	0.21440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ecq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.700	42.700	1.890
High resolution limit [Å]	1.780	5.300	1.780
Rmerge	0.074	0.025	1.456
Rmeas	0.082	0.027	1.607
Total number of observations	490415
Number of reflections	85301	3222	13651
<I/σ(I)>	13.64	50.35	1.09
Completeness [%]	99.8	99.8	99
Redundancy	5.749	5.678	5.657
CC(1/2)	0.999	0.999	0.445

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	10% PEG 2000MME, 0.1 M MES