7U7T

Human DNA polymerase eta-DNA-dGMPNPP ternary mismatch complex in 0.05 mM Mg2+ for 600s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-03-27
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9786
Spacegroup name	P 61
Unit cell lengths	98.631, 98.631, 81.973
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.710 - 1.550
R-factor	0.1889
Rwork	0.188
R-free	0.20220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ecq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.710	42.710	1.650
High resolution limit [Å]	1.550	4.640	1.550
Rmerge	0.051	0.032	0.851
Rmeas	0.056	0.035	0.963
Total number of observations	694913
Number of reflections	64445	4805	18718
<I/σ(I)>	17.36	52.66	1.51
Completeness [%]	97.3	99.6	90.1
Redundancy	5.564	5.677	4.241
CC(1/2)	0.999	0.999	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	10% PEG 2000MME, 0.1 M MES