7U7S
Human DNA polymerase eta-DNA-dGMPNPP ternary mismatch complex in 0.025 mM Mg2+ for 600s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-03-27 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9786 |
Spacegroup name | P 61 |
Unit cell lengths | 98.452, 98.452, 82.203 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.630 - 1.600 |
R-factor | 0.189 |
Rwork | 0.188 |
R-free | 0.20740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ecq |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.630 | 42.630 | 1.690 |
High resolution limit [Å] | 1.600 | 4.770 | 1.600 |
Rmerge | 0.050 | 0.024 | 1.110 |
Rmeas | 0.055 | 0.026 | 1.255 |
Total number of observations | 656691 | ||
Number of reflections | 59887 | 4438 | 18935 |
<I/σ(I)> | 17.28 | 59.28 | 1.23 |
Completeness [%] | 99.8 | 99.7 | 99 |
Redundancy | 5.565 | 5.678 | 4.618 |
CC(1/2) | 0.999 | 0.999 | 0.452 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% PEG 2000MME, 0.1 M MES |