7U7J

Human DNA polymerase eta-DNA ternary mismatch complex:reaction with 10.0 mM Mn2+ for 300s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-12-14
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9787
Spacegroup name	P 61
Unit cell lengths	98.249, 98.249, 82.161
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.540 - 1.580
R-factor	0.1835
Rwork	0.181
R-free	0.21290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kfk
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.540	42.540	1.680
High resolution limit [Å]	1.580	4.720	1.580
Rmerge	0.053	0.026	0.962
Rmeas	0.058	0.028	1.101
Total number of observations	661751
Number of reflections	61300	4534	19394
<I/σ(I)>	16.74	52.33	1.45
Completeness [%]	99.8	99.8	99.1
Redundancy	5.477	5.609	4.251
CC(1/2)	0.999	0.999	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	10% PEG 2000MME, 0.1 M MES