7U77
Human DNA polymerase eta-DNA ternary mismatch complex:reaction with 1.0 mM Mg2+ for 40s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-11-28 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9787 |
Spacegroup name | P 61 |
Unit cell lengths | 98.219, 98.219, 82.291 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.530 - 1.580 |
R-factor | 0.1849 |
Rwork | 0.184 |
R-free | 0.20260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ecq |
RMSD bond length | 0.009 |
RMSD bond angle | 1.221 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.530 | 42.530 | 1.680 |
High resolution limit [Å] | 1.580 | 4.710 | 1.580 |
Rmerge | 0.044 | 0.025 | 0.823 |
Rmeas | 0.049 | 0.027 | 0.929 |
Total number of observations | 667368 | ||
Number of reflections | 120048 | 4561 | 18159 |
<I/σ(I)> | 18.6 | 56.15 | 1.62 |
Completeness [%] | 98.5 | 99.5 | 92.2 |
Redundancy | 5.559 | 5.622 | 4.565 |
CC(1/2) | 0.999 | 0.999 | 0.597 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% PEG 2000MME, 0.1 M MES |