7U74

Human DNA polymerase eta-DNA ternary mismatch complex:reaction with 0.5 mM Mn2+ for 1800s then with 10 mM Mn2+ for 30s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-04-15
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9786
Spacegroup name	P 61
Unit cell lengths	98.675, 98.675, 82.213
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.300 - 1.520
R-factor	0.1924
Rwork	0.190
R-free	0.22450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kff
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.300	32.300	1.610
High resolution limit [Å]	1.520	4.530	1.520
Rmerge	0.034	0.020	0.659
Rmeas	0.038	0.022	0.786
Total number of observations	714176
Number of reflections	69928	5196	21464
<I/σ(I)>	21.79	66.07	1.51
Completeness [%]	99.2	99.5	96
Redundancy	5.207	5.619	3.146
CC(1/2)	1.000	0.999	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	10% PEG 2000MME, 0.1 M MES