7U4F

Neuraminidase from influenza virus A/Moscow/10/1999(H3N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9786
Spacegroup name	I 4 2 2
Unit cell lengths	136.153, 136.153, 150.767
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.500 - 1.400
R-factor	0.1549
Rwork	0.154
R-free	0.16510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aep
RMSD bond length	0.018
RMSD bond angle	2.048
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.500	1.401
High resolution limit [Å]	1.397	1.397
Rmerge	0.108	1.260
Rmeas	0.113
Number of reflections	138645	138645
<I/σ(I)>	20.2	2.2
Completeness [%]	99.9
Redundancy	23
CC(1/2)		0.674

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	2.0 M NH4-sulfate, 0.1 M acetate pH 4.6