7U34

The structure of phosphoglucose isomerase from Aspergillus fumigatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-11
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 43 21 2
Unit cell lengths	84.452, 84.452, 232.024
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.010 - 1.560
R-factor	0.1586
Rwork	0.157
R-free	0.18320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7oyl
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.359	1.745
High resolution limit [Å]	1.560	1.560
Number of reflections	71374	3567
<I/σ(I)>	19.7
Completeness [%]	95.8
Redundancy	26.4
CC(1/2)	1.000	0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	1.4 M sodium citrate dihydrate, 0.1 M Hepes-NaOH pH 7.5