7U2V

Plasmodium falciparum Cyt c2 DSD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.73816
Spacegroup name	P 1 21 1
Unit cell lengths	40.846, 54.613, 71.464
Unit cell angles	90.00, 102.19, 90.00

Refinement procedure

Resolution	39.920 - 2.550
R-factor	0.1948
Rwork	0.189
R-free	0.24310
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	SOLVE
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.920	39.920	2.660
High resolution limit [Å]	2.550	8.830	2.550
Rmerge	0.410	0.095	3.162
Rmeas	0.416	0.096	3.223
Rpim	0.064	0.013	0.609
Total number of observations	407151	12521	12083
Number of reflections	8552	257	415
<I/σ(I)>	20.2	28.5	8
Completeness [%]	84.2	97	35.1
Redundancy	47.6	48.7	29.1
CC(1/2)	0.979	0.998	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	286	0.2 M sodium chloride, 0.1 M Bis-Tris, pH 5.5, 25% PEG3350