7U25

Crystal structure of arabidopsis thaliana acetohydroxyacid synthase W574L mutant in complex with bispyribac-sodium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9537
Spacegroup name	P 64 2 2
Unit cell lengths	179.952, 179.952, 185.472
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.430 - 3.190
R-factor	0.1748
Rwork	0.173
R-free	0.19240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k2o
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.430	3.390
High resolution limit [Å]	3.190	3.190
Number of reflections	29873	4587
<I/σ(I)>	13.3
Completeness [%]	99.4
Redundancy	15.4
CC(1/2)	0.998	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.4	293	CHES buffer, potassium sodium tartrate, ammonium sulfate