7U1Y
Structure of SPAC806.04c protein from fission yeast bound to AlF4 and Co2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-09 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.605 |
| Spacegroup name | I 4 |
| Unit cell lengths | 133.721, 133.721, 56.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.280 - 1.810 |
| R-factor | 0.1574 |
| Rwork | 0.156 |
| R-free | 0.18180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7u1v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.978 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.280 | 1.850 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rpim | 0.021 | 0.144 |
| Number of reflections | 43687 | 1572 |
| <I/σ(I)> | 26.6 | 4.1 |
| Completeness [%] | 95.9 | 59 |
| Redundancy | 11.4 | 4.4 |
| CC(1/2) | 0.999 | 0.925 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 0.2M NaCL, 0.1 M HEPES-NaOH (pH 7.0), 20% (w/v) PEG 6,000; cryprotectant: 0.2M NaCL, 0.1 M HEPES-NaOH (pH 7.0), 22% (w/v) PEG 6,000, 25% (w/v) sucrose |
