Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-08-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | F 2 3 |
Unit cell lengths | 269.155, 269.155, 269.155 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 134.941 - 2.701 |
Rwork | 0.215 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uik |
RMSD bond length | 0.009 |
RMSD bond angle | 1.768 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 134.941 | 2.810 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.127 | 0.554 |
Rmeas | 0.151 | 0.663 |
Rpim | 0.081 | 0.359 |
Number of reflections | 44137 | 4997 |
<I/σ(I)> | 8.9 | |
Completeness [%] | 99.8 | |
Redundancy | 5.5 | 5.7 |
CC(1/2) | 0.995 | 0.811 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.15M Ammonium Acetate, 25% PEG3350, 0.05M Bis Tris |