7U0N

Crystal structure of chimeric omicron RBD (strain BA.1) complexed with human ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-11
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	81.130, 120.179, 113.325
Unit cell angles	90.00, 92.44, 90.00

Refinement procedure

Resolution	30.110 - 2.610
R-factor	0.1906
Rwork	0.188
R-free	0.23500
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2ajf
RMSD bond length	0.002
RMSD bond angle	0.557
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	113.000	2.680
High resolution limit [Å]	2.610	2.610
Rmerge	0.055
Number of reflections	61585	110
<I/σ(I)>	9.3
Completeness [%]	92.6
Redundancy	4
CC(1/2)	0.999	0.689

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	100 mM Tris, pH 8.5, 20% PEG6000, 100 mM sodium chloride