7U08

Structure of CD148 fibronectin type III domain 1 and 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97850
Spacegroup name	P 65 2 2
Unit cell lengths	142.325, 142.325, 57.722
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.737 - 3.307
R-factor	0.270966472295
Rwork	0.268
R-free	0.30026
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.830
Data reduction software	HKL-3000
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.360
High resolution limit [Å]	3.300	3.300
Rmerge	0.158
Number of reflections	5502	258
<I/σ(I)>	34.3
Completeness [%]	100.0
Redundancy	38.8
CC(1/2)		0.235

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.25M MgCl2, 0.1M HEPES pH7.5, 25% PEG3350