7U04

IOMA class antibody ACS101

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-24
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97741
Spacegroup name	P 32 2 1
Unit cell lengths	67.130, 67.130, 199.293
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.750 - 2.310
R-factor	0.2189
Rwork	0.217
R-free	0.25620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d2p
RMSD bond length	0.002
RMSD bond angle	0.577
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.750	2.340
High resolution limit [Å]	2.310	2.310
Rmerge	0.129	1.220
Rmeas	0.136	1.320
Rpim	0.043	0.490
Number of reflections	23788	1113
<I/σ(I)>	19.3	1.5
Completeness [%]	100.0	100
Redundancy	10	7.1
CC(1/2)	0.880	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	10% (v/v) glycerol, 0.1M of HEPES (pH 7.5), 5% (w/v) polyethylene glycol 3000 and 30% (v/v) polyethylene glycol 400