7TYE
The crystal structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase mutant (G108S) from E. Coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.990, 108.514, 47.438 |
| Unit cell angles | 90.00, 98.74, 90.00 |
Refinement procedure
| Resolution | 45.200 - 1.940 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g57 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.626 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.200 | 1.970 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.044 | 0.949 |
| Rmeas | 0.052 | 1.121 |
| Rpim | 0.026 | 0.694 |
| Number of reflections | 59935 | 3026 |
| <I/σ(I)> | 34.87 | 1.1 |
| Completeness [%] | 98.4 | 98.3 |
| Redundancy | 3.8 | 3.2 |
| CC(1/2) | 0.993 | 0.694 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 286 | 0.2 M Ammonium Formate, 20 % (w/v) PEG 3350 |
