7TY1

Crystal structure of apo eosinophil cationic protein (ribonuclease 3) from Macaca fascicularis (MfECP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.23985
Spacegroup name	P 21 21 21
Unit cell lengths	37.330, 39.270, 76.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.010 - 1.800
R-factor	0.2109
Rwork	0.207
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qmt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.020	38.020	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.161	0.070	0.780
Rmeas	0.169	0.073	0.814
Rpim	0.049	0.022	0.227
Number of reflections	10862	478	1076
<I/σ(I)>	14.2	29.1	3.6
Completeness [%]	100.0	100	100
Redundancy	12	10.3	12.8
CC(1/2)	0.998	0.998	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	298	0.1M Citric acid pH 3.5, 25% PEG 3350