7TXO

Selenomethionine Labeled Structure of RexT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2020-08-08
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	P 64
Unit cell lengths	100.460, 100.460, 36.048
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.500 - 2.500
R-factor	0.2325
Rwork	0.230
R-free	0.25940
Structure solution method	AB INITIO PHASING
RMSD bond length	0.012
RMSD bond angle	1.707
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.500	2.650
High resolution limit [Å]	2.500	2.500
Rmerge	0.157	1.193
Number of reflections	7429	2273
<I/σ(I)>	12.78
Completeness [%]	99.7
Redundancy	8.8
CC(1/2)	0.998	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10 mM MES, pH 6.3, 0.2 M ammonium chloride, 20% v/v PEG3350, 5% v/v glycerol