7TXN

Reduced Structure of RexT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-15
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 64
Unit cell lengths	99.891, 99.891, 36.472
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.610 - 1.950
R-factor	0.1839
Rwork	0.180
R-free	0.22180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7txo
RMSD bond length	0.010
RMSD bond angle	1.185
Data reduction software	XDS
Data scaling software	XDS (1.19.2_4158)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.610	2.070
High resolution limit [Å]	1.950	1.950
Rmerge	0.150	1.045
Number of reflections	15092	2118
<I/σ(I)>	18.44
Completeness [%]	97.6
Redundancy	13.7
CC(1/2)	0.999	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10 mM MES, pH 6.3, 0.2 M ammonium chloride, 20% v/v PEG3350, 5% v/v glycerol