7TX8

Long form D7 protein from Anopheles darlingi with U46619 and serotonin bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 31 2 1
Unit cell lengths	98.060, 98.060, 273.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.310 - 2.510
R-factor	0.2202
Rwork	0.218
R-free	0.26610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	unliganded AnDar-D7L2
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	53.310	53.310	2.580
High resolution limit [Å]	2.510	11.230	2.510
Rmerge	0.084	0.056	0.639
Rmeas	0.086	0.058	0.653
Total number of observations	1174205
Number of reflections	53228	698	3889
<I/σ(I)>	25.46	45.59	6.12
Completeness [%]	100.0	99.6	100
Redundancy	22.06	18.239	23.12
CC(1/2)	0.999	0.999	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.2 M ammonium sulfate, 30% PEG 5000 MME, 0.1 M HEPES, pH 7.5