7TWM
Structure of a borosin methyltransferase from Mycena rosella with peptide CspL(MroMCspL) in complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-07-09 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.978720 |
| Spacegroup name | P 65 |
| Unit cell lengths | 190.960, 190.960, 93.589 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.930 |
| R-factor | 0.1753 |
| Rwork | 0.174 |
| R-free | 0.20190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7twk |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.141 |
| Data reduction software | autoPROC (1.0.5) |
| Data scaling software | autoPROC (1.0.5) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.700 | 1.940 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.109 | 1.310 |
| Number of reflections | 145206 | 1457 |
| <I/σ(I)> | 18.9 | 1.8 |
| Completeness [%] | 99.5 | 94 |
| Redundancy | 13.7 | |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 282 | 25% PEG 4000, and 100 mM sodium cacodylate |
