7TUG
Crystal structure of Tapasin in complex with PaSta2-Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2021-06-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.090, 168.820, 108.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.330 - 3.900 |
| R-factor | 0.2846 |
| Rwork | 0.282 |
| R-free | 0.33190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f8u |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.507 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.330 | 4.040 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.107 | |
| Rmeas | 0.127 | |
| Rpim | 0.065 | 0.851 |
| Number of reflections | 8241 | 692 |
| <I/σ(I)> | 7.5 | 0.85 |
| Completeness [%] | 95.4 | 80.75 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.998 | 0.477 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 17% PEG 10000, 0.1M Bis-Tris, 0.1M Am Acetate |
