7TSX

Structure of Enterobacter cloacae Cap2 bound to CdnD02 C-terminus, Apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-22
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	50.262, 76.546, 127.631
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.650 - 1.770
R-factor	0.1706
Rwork	0.170
R-free	0.19150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7to3
RMSD bond length	0.006
RMSD bond angle	0.676
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	127.630	1.800
High resolution limit [Å]	1.770	1.770
Rmerge	0.087	1.315
Rpim	0.036	0.548
Number of reflections	49001	2751
<I/σ(I)>	14.1	1.3
Completeness [%]	99.9	98.7
Redundancy	6.7	6.5
CC(1/2)	0.999	0.606

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Tris-HCl pH 8.5, 0.8 M LiCl, and 25% PEG 3350