7TRV
Crystal Structure of the DNA-Binding Domain of the LysR family Transcriptional Regulator YfbA from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.943, 146.270, 33.701 |
| Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
| Resolution | 32.840 - 1.800 |
| R-factor | 0.1957 |
| Rwork | 0.187 |
| R-free | 0.23200 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.383 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.141 | 0.957 |
| Rmeas | 0.158 | 1.111 |
| Rpim | 0.071 | 0.552 |
| Number of reflections | 27328 | 1352 |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 99.2 | 98 |
| Redundancy | 4.8 | |
| CC(1/2) | 0.599 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.025 M Magnesium sulfate, 0.05 M TRIS HCl pH 8.5, 1.8 M Ammonium sulfate |
