7TQW

Kod RSGA incorporating PMT, n+2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-20
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	64.136, 111.522, 144.268
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.036 - 3.010
R-factor	0.1856
Rwork	0.176
R-free	0.27060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rsr
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.480	2.296
High resolution limit [Å]	2.216	2.216
Rmerge	0.246	2.354
Rmeas	0.348	3.329
Rpim	0.246	2.354
Number of reflections	47878	4637
<I/σ(I)>	2.52
Completeness [%]	85.1	48.68
Redundancy	1.8	1.7
CC(1/2)	0.956	0.362

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 8K, MOPS, magnesium acetate