7TQR
Crystal Structure of histidine ammonia lyase from Thermoplasma acidophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-12-08 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 168.990, 168.990, 67.751 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.860 - 2.100 |
| R-factor | 0.1496 |
| Rwork | 0.148 |
| R-free | 0.18270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gk3 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.452 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.480 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 33715 | 2714 |
| <I/σ(I)> | 19.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 18.1 | |
| CC(1/2) | 0.999 | 0.961 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM sodium cacodylate pH 7.0, 50 mM lithium chloride, 12 mM spermine tetrahydrochloride, 52 mM strontium chloride, and 30% v/v (+/-)-2-methyl-2,4-pentanediol |
