7TQN

Structure of human TREX1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-E
Synchrotron site	APS
Beamline	21-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-04-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	57.081, 57.081, 123.117
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.860 - 1.800
R-factor	0.1783
Rwork	0.175
R-free	0.20760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mxj
RMSD bond length	0.008
RMSD bond angle	0.946
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.860	1.840
High resolution limit [Å]	1.800	1.800
Rpim	0.044	0.798
Number of reflections	19529	1133
<I/σ(I)>	10.2	1.5
Completeness [%]	99.5
Redundancy	13.1
CC(1/2)	0.996	0.488

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Sodium chloride, 0.1 M Bis-Tris pH 5.5, 20% PEG-3350