7TNG

Kringle domain of human Receptor Tyrosine Kinase-Like Orphan Receptor 1 (ROR1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-30
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 32 2 1
Unit cell lengths	48.157, 48.157, 69.021
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.710 - 1.400
R-factor	0.21127
Rwork	0.211
R-free	0.21394
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ba5 6osn
RMSD bond length	0.018
RMSD bond angle	2.158
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.710	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.101	0.808
Rpim	0.063	0.494
Number of reflections	18755	938
<I/σ(I)>	18.3	1.7
Completeness [%]	99.8
Redundancy	7.4
CC(1/2)	0.985	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	0.8-1.2 M Sodium citrate tribasic dihydrate, 0.1 M sodium cacodylate, pH 6.5