Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.470, 104.175, 144.328 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.678 - 1.790 |
| Rwork | 0.153 |
| R-free | 0.18590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6myw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.592 |
| Data reduction software | XDS (BUILT=20180808) |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER (2.8.1) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.680 | 1.830 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.147 | 1.390 |
| Rmeas | 0.159 | 1.513 |
| Rpim | 0.060 | 0.589 |
| Number of reflections | 33499 | 2315 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 99.8 | 97.3 |
| Redundancy | 13.3 | |
| CC(1/2) | 0.998 | 0.745 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | Crystal Screen #20: 0.2 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6, 25% w/v Polyethylene glycol 4,000 Protein dissolved in 100 mM KPi, pH 7.5 |
