7TMY

Structure of Mouse Importin alpha NEIL3 NLS Peptide Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-27
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.45
Spacegroup name	P 21 21 21
Unit cell lengths	78.789, 89.695, 99.383
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.030 - 2.210
R-factor	0.1872
Rwork	0.186
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ul1
RMSD bond length	0.006
RMSD bond angle	0.855
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.030	2.290
High resolution limit [Å]	2.210	2.210
Rmerge	0.183	2.530
Number of reflections	67874	3511
<I/σ(I)>	11.82	0.94
Completeness [%]	99.6	98.7
Redundancy	12.8	12.7
CC(1/2)	1.000	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	sodium citrate buffer (pH 6.0), sodium citrate and DTT