7TMU
Crystal Structure of the Protein of Unknown Function YPO0625 from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97932 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.872, 92.935, 82.991 |
| Unit cell angles | 90.00, 92.05, 90.00 |
Refinement procedure
| Resolution | 37.870 - 2.550 |
| R-factor | 0.1953 |
| Rwork | 0.193 |
| R-free | 0.23420 |
| Structure solution method | SAD |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.418 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.081 | 0.701 |
| Rmeas | 0.095 | 0.838 |
| Rpim | 0.049 | 0.451 |
| Number of reflections | 29739 | 1078 |
| <I/σ(I)> | 20.4 | 1.47 |
| Completeness [%] | 96.5 | 69.5 |
| Redundancy | 3.4 | 2.6 |
| CC(1/2) | 1.000 | 0.665 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1 M Hepes pH 7.5, 10 %(w/v) PEG 8000,10 %(w/v) ethylene glycol |
