7TLX

Crystal Structure of cytochrome c from Pseudomonas putida S16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2020-09-25
Detector	RAYONIX MX300-HS
Wavelength(s)	0.9787
Spacegroup name	C 1 2 1
Unit cell lengths	79.730, 30.170, 49.100
Unit cell angles	90.00, 121.56, 90.00

Refinement procedure

Resolution	41.840 - 1.900
R-factor	0.1526
Rwork	0.151
R-free	0.17470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1crc
RMSD bond length	0.010
RMSD bond angle	1.134
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.840	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	8051	527
<I/σ(I)>	19.7
Completeness [%]	100.0
Redundancy	3.6
CC(1/2)	0.996	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MES monohydrate pH 6.0, 20% w/v Polyethylene glycol monomethyl ether 2000