7TLF

Structure of the photoacclimated Light Harvesting Complex PE545 from Proteomonas sulcata

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979493
Spacegroup name	P 1 21 1
Unit cell lengths	88.249, 132.237, 93.413
Unit cell angles	90.00, 116.92, 90.00

Refinement procedure

Resolution	29.350 - 2.800
R-factor	0.2483
Rwork	0.245
R-free	0.30580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7tja
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.350	29.350	2.880
High resolution limit [Å]	2.780	10.760	2.780
Rmerge	0.240	0.079	0.904
Rmeas	0.267	0.089	1.012
Rpim	0.115	0.040	0.444
Total number of observations	239812	3727	17890
Number of reflections	46887	811	3661
<I/σ(I)>	5	10.5	1.6
Completeness [%]	97.2	94.5	77.2
Redundancy	5.1	4.6	4.9
CC(1/2)	0.989	0.994	0.739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% PEG 3350, 50 mM HEPES, 100 mM MgCl2, and 15% glycerol.