7TL6

Murine meteorin N-terminal CUB domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	48.508, 48.508, 191.542
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.760 - 2.300
R-factor	0.2537
Rwork	0.252
R-free	0.29190
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	autoPROC
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.900	2.380
High resolution limit [Å]	2.300	2.300
Rpim	0.037	0.640
Number of reflections	10896	1013
<I/σ(I)>	17.5	1.2
Completeness [%]	99.8	97.5
Redundancy	24	12.29
CC(1/2)	0.986	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	100 mM Tris-HCl, pH 8.5, 200 mM lithium sulfate, 31% w/v PEG3350