7TL3

Crystal Structure of Yeast p58C Multi-Tyrosine Mutant 5YF431

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-20
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	41.044, 51.097, 90.223
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.359 - 2.066
R-factor	0.2397
Rwork	0.236
R-free	0.27670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dtz
RMSD bond length	0.003
RMSD bond angle	0.494
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.359	30.000	2.120
High resolution limit [Å]	2.050	4.410	2.050
Rmerge	0.146	0.104	0.473
Rmeas	0.166	0.122	0.549
Rpim	0.078	0.061	0.272
Number of reflections	11722	1242	1011
<I/σ(I)>	6.8
Completeness [%]	96.8	93.7	85
Redundancy	4.8	4.5	3.4
CC(1/2)		0.979	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Protein drops contained 20 mM HEPES (pH 6.8), 75 mM NaCl, 2 mM DTT mixed with equal volume of 100 mM TRIS (pH 8.5) and 55-70% MPD.