7TL2

Crystal Structure of Yeast p58C Multi-Tyrosine Mutant 5YF412

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-06
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	40.624, 51.870, 89.479
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.018 - 1.529
R-factor	0.1524
Rwork	0.152
R-free	0.16800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dtz
RMSD bond length	0.014
RMSD bond angle	1.207
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.580
High resolution limit [Å]	1.529	3.290	1.530
Rmerge	0.040	0.026	0.353
Rmeas	0.043	0.028	0.392
Rpim	0.018	0.012	0.169
Number of reflections	29249	3123	2801
<I/σ(I)>	15.2
Completeness [%]	99.8	99.4	98.1
Redundancy	5.9	5.7	5.1
CC(1/2)		0.999	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Protein drops contained 20 mM HEPES (pH 6.8), 75 mM NaCl, 2 mM DTT mixed with equal volume of 100 mM TRIS (pH 8.5) and 55-70% MPD.