7TJM

Bacteriophage Q beta capsid protein in T3 symmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	H 3
Unit cell lengths	286.627, 286.627, 662.678
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.560 - 3.540
R-factor	0.2806
Rwork	0.278
R-free	0.32780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qbe
RMSD bond length	0.007
RMSD bond angle	1.975
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.560	3.620
High resolution limit [Å]	3.540	3.540
Rmerge	0.150
Number of reflections	247697	17430
<I/σ(I)>	6.4
Completeness [%]	99.9
Redundancy	10
CC(1/2)	0.800	0.150

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	298	PEG 3350, cobalt chloride, Tris