7TJD

Bacteriophage Q beta capsid protein in T1 symmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979
Spacegroup name	H 3
Unit cell lengths	271.926, 271.926, 166.564
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.070 - 3.500
R-factor	0.2011
Rwork	0.198
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qbe
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.100	3.623
High resolution limit [Å]	3.500	3.500
Rmerge	0.120
Number of reflections	57858	5795
<I/σ(I)>	9.7
Completeness [%]	99.7
Redundancy	2.1
CC(1/2)		0.200

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	PEG 3350, cobalt chloride, Tris