7TI0

Structure of CTX-M-15 bound to RPX-7063 at 1.5A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9765
Spacegroup name	P 21 21 21
Unit cell lengths	45.880, 44.780, 117.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.500
R-factor	0.16
Rwork	0.160
R-free	0.17800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yms
RMSD bond length	0.014
RMSD bond angle	1.858
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.540
High resolution limit [Å]	1.500	6.710	1.500
Rmerge	0.060	0.027	0.229
Number of reflections	39211	493	2862
<I/σ(I)>	18.79	38.4	6.1
Completeness [%]	99.2	95.5	99.3
Redundancy	3.97	3.6	3.87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.1M bis-tris propane pH 7.0, 2.3M Ammonium sulfate